Figure 3From: Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)Detection of loxP fragment excision by PCR. Genomic DNA samples from the putative marker-free T1 plants L10-T1-10 and L10-T1-18 were amplified by PCR for detection of Cre/loxP-mediated loxP fragment excision using primer pairs P1/P2, P3/P4 and P1/P4 [34]. The presence or absence of the Hpt and Cry1Ab/1Ac genes were also detected by PCR. Plasmid DNA of pCCreloxPBt and genomic DNA from MD44 were used as the controls. Primers are listed in Table 4.Back to article page