Expression of the Cry1Ab/1Ac gene in transgenic J. curcas plants. (A) Detection of Cry1Ab/1Ac gene transcripts by northern blot analysis. The upper panel shows the result of northern blot analysis detected by a Cry1Ab/1Ac probe. The lower panel is the image of RNA loading assessed by methylene blue staining. MD44, J. curcas cultivar MD44; L10-T1-10 and L10-T1-18, transgenic T1 plants. (B) Detection of Cry1Ab/1Ac gene transcripts by qRT-PCR. Results are shown as the normalized expression with the expression level of the Cry1Ab/1Ac gene in L10-T1-18 set as 1. The experiments were performed in triplicate and the data are presented as means ± SD.