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Figure 5 | Biotechnology for Biofuels

Figure 5

From: Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)

Figure 5

Detection of Cry1Ab/1Ac proteins in transgenic J. curcas plants. The Cry1Ab/1Ac proteins were detected with anti-CRY1Ab polyclonal antibodies. The Cry1Ab/1Ac proteins expressed in L24 of transgenic rice were used as the positive control. The proteins stained with Coomassie blue in duplicate SDS-PAGE gel serve as protein loading controls (left panel). Arrow indicates the position of Cry1Ab/1Ac. MD44, J. curcas cultivar MD44; L10-T1-10 and L10-T1-18, transgenic T1 plants; L24 (Rice), transgenic rice carrying the P Pepc :Cry1Ab/1Ac:T Nos gene [34].

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