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Table 1 PCR analysis of the T 0 transgenic plants

From: Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)

T0plant Cry1Ab/1Ac 1 P1-P42 Hpt 3
pCCreloxPBt4 + - +
MD44 - - -
1 + - -
2 + - +
2B + - +
4A + - +
4B + - +
6 + - +
8 + - +
10 + + +
11 + + +
12 + - +
13 + - +
19 + - +
20 + + -
21 + + +
24 + - +
33 + + +
37 + + +
38 + + +
39 + - +
40 + - +
  1. 1The primers for detecting the presence of the Cry1Ab/1Ac gene were Bt F1 and Bt R1; 2the primers for detecting the excision of the loxP fragment or the presence of P1-P4 fragment were P1 and P4 [34]; 3the primers for detecting the presence of the Hpt gene were Hpt F and Hpt910-1; 4binary construct used for transgenic J. curcas production [34].
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