Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)

Table 2 PCR analysis of the L10 T ₁ transgenic plants

T₁plant	P1-P2¹	P3-P4²	P1-P4³	Hpt ⁴	Cry1Ab/1Ac ⁵
pCCreloxPBt⁶	+	+	-	+	+
MD44	-	-	-	-	-
L10-T₁-1	-	+	-	+	+
L10-T₁-2	-	-	+	+	+
L10-T₁-3	-	+	-	+	+
L10-T₁-4	-	+	+	+	+
L10-T₁-5	-	-	+	-	+
L10-T₁-6	-	+	-	+	+
L10-T₁-7	-	+	-	+	+
L10-T₁-8	-	+	-	+	+
L10-T₁-9	-	+	-	+	+
L10-T₁-10	-	-	+	-	+
L10-T₁-11	-	+	+	+	+
L10-T₁-12	-	+	-	+	+
L10-T₁-13	-	+	-	+	+
L10-T₁-14	-	-	-	-	-
L10-T₁-15	-	+	+	+	+
L10-T₁-16	-	+	+	+	+
L10-T₁-17	-	+	+	+	+
L10-T₁-18	-	-	+	-	+
L10-T₁-19	-	+	+	+	+
L10-T₁-20	-	+	+	+	+

¹The primers for detecting the presence of P1-P2 fragment were P1 and P2 [34]; ²the primers for detecting the presence of P3-P4 fragment were P3 and P4 [34]; ³the primers for detecting the excision of the loxP fragment or the presence of P1-P4 fragment were P1 and P4 [34]; ⁴the primers for detecting the presence of the Hpt gene were Hpt F and Hpt910-1; ⁵the primers for detecting the presence of the Cry1Ab/1Ac gene were Bt F1 and Bt R1; ⁶binary construct used for transgenic J. curcas production [34].

ISSN: 2731-3654