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Table 2 PCR analysis of the L10 T 1 transgenic plants

From: Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)

T1plant P1-P21 P3-P42 P1-P43 Hpt 4 Cry1Ab/1Ac 5
pCCreloxPBt6 + + - + +
MD44 - - - - -
L10-T1-1 - + - + +
L10-T1-2 - - + + +
L10-T1-3 - + - + +
L10-T1-4 - + + + +
L10-T1-5 - - + - +
L10-T1-6 - + - + +
L10-T1-7 - + - + +
L10-T1-8 - + - + +
L10-T1-9 - + - + +
L10-T1-10 - - + - +
L10-T1-11 - + + + +
L10-T1-12 - + - + +
L10-T1-13 - + - + +
L10-T1-14 - - - - -
L10-T1-15 - + + + +
L10-T1-16 - + + + +
L10-T1-17 - + + + +
L10-T1-18 - - + - +
L10-T1-19 - + + + +
L10-T1-20 - + + + +
  1. 1The primers for detecting the presence of P1-P2 fragment were P1 and P2 [34]; 2the primers for detecting the presence of P3-P4 fragment were P3 and P4 [34]; 3the primers for detecting the excision of the loxP fragment or the presence of P1-P4 fragment were P1 and P4 [34]; 4the primers for detecting the presence of the Hpt gene were Hpt F and Hpt910-1; 5the primers for detecting the presence of the Cry1Ab/1Ac gene were Bt F1 and Bt R1; 6binary construct used for transgenic J. curcas production [34].