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Table 3 Bioassay using artificial infestation of A. micaceanus larvae on leaf explants of MD44 and transgenic plant L10-T 1 -10

From: Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis δ-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)

Plant

Larvae upon infestation, number

Larvae or pupa after infestation, number

Mortality %1

Experiment I

   

MD44

5

4 larvae + 1 pupa

0

L10-T1-10

5

1 pupa

80%

Experiment II

   

MD44

5

5 larvae

0

L10-T1-10

5

0

100%

Experiment III

   

MD44

5

5 larvae

0

L10-T1-10

5

0

100%

  1. 1Viability and mortality of insects were scored at 4 days after infestation.