Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels

Table 2 FAEE-producing strains of E. coli

Gene deletion	Wax exter synthase and alcohol production	Overexpression of other genes	Yield (g l^-1)	Process time (h)	Productivity (g l^-1h^-1)	Process	Reference
-	atfA; pdc; adhB		0.43	48	0.009	batch + oleate	[24]
-	atfA; pdc; adhB		1.28	72	0.018	fed-batch + oleate	[24]
-	atfA; pdc; adhB		11	47	0.234	fed-batch + oleate	[222]
-	atfA; ethanol added	tesA; fadD	0.1	48	0.002	batch	[26]
fadE	atfA; ethanol added	tesA; fadD	0.4	48	0.008	batch	[26]
fadE	atfA; pdc; adhB	tesA	0.037	48	0.001	batch	[26]
fadE	atfA; pdc; adhB	tesA; fadD	0.233	48	0.005	batch	[26]
fadE	atfA; pdc; adhB	tesA; fadD; atfA	0.427	48	0.009	batch	[26]
fadE	atfA; pdc; adhB	tesA; fadD; atfA	0.674	48	0.014	batch with dodecane overlay	[26]
fadE	atfA; pdc; adhB	tesA; fadD; accABCD	0.922	72	0.013	fed-batch	[223]
fadE	atfA; pdc; adhB (1)	tesA; fadD; fadR (1)	1.5	72	0.021	batch	[191]

(1) Dodecane overlay to prevent fatty acid ethyl ester (FAEE) evaporation. Gene source: Acinetobacter baylyi ADP1 (atfA); Zymomonas mobilis (pdc; adhB); Escherichia coli (all other genes).

ISSN: 2731-3654