Studying the rapid bioconversion of lignocellulosic sugars into ethanol using high cell density fermentations with cell recycle

Table 1 Traditional and RaBIT fermentation comparison

	Traditional ferm. specific xylose cons. rate^+,a, g/g/h	48 h traditional fermentation EtOH prod.^*,b, g/L/h	120 h traditional fermentation EtOH prod.^*,c, g/L/h	Average RaBIT fermentation EtOH prod.^*,d, g/L/h	Traditional fermentation EtOH conc., g/L	Average RaBIT fermentation EtOH conc., g/L
Strain
Y73	0.022 ± 0.001	0.604 ± 0.001	0.271 ± 0.001	1.315 ± 0.017	32.5 ± 0.1	31.6 ± 0.4
Y127	0.015 ± 0.003	0.603 ± 0.007	0.250 ± 0.001	1.346 ± 0.014	30.0 ± 0.2	32.3 ± 0.3
Y128	0.077 ± 0.003	0.720 ± 0.006	0.322 ± 0.001	1.808 ± 0.045	38.6 ± 0.1	43.4 ± 1.1
424A	0.107 ± 0.001	0.752 ± 0.002	0.345 ± 0.003	1.694 ± 0.005	41.3 ± 0.3	40.7 ± 0.1
8b	0.650 ± 0.011	0.856 ± 0.004	0.356 ± 0.001	1.808 ± 0.021	42.7 ± 0.01	43.4 ± 0.5
KO11	0.018 ± 0.003	0.416 ± 0.001	0.205 ± 0.000	1.319 ± 0.026	24.6 ± 0.0	31.6 ± 0.6

⁺Specific xylose consumption rate was calculated by dividing the xylose consumed by the time period and average dry cell weight concentration as correlated from OD measurements; ^*ethanol productivity was calculated by dividing the ethanol concentration by time of fermentation;
calculated from ^a24 to 48 h, ^b0 to 48 h, ^c0 to 120 h, or ^d0 to 24 h;
average RaBIT fermentation calculations were performed by averaging the data from the two cycles.

ISSN: 2731-3654