Studying the rapid bioconversion of lignocellulosic sugars into ethanol using high cell density fermentations with cell recycle

Table 2 RaBIT fermentation cellular rates

	Specific xylose cons. rate^+,a, g/g/h	Average viable cell density^a, g/L DCW	Growth rate^b, g/L/h	Death rate^c, g/L/h
No nutrients
Cycle 1	0.092 ± 0.005	12.3 ± 0.5	0.401 ± 0.113	-0.156 ± 0.024
Cycle 2	0.084 ± 0.001	12.8 ± 0.5	0.624 ± 0.356	-0.176 ± 0.028
Cycle 3	0.088 ± 0.006	12.7 ± 0.9	0.547 ± 0.383	-0.176 ± 0.050
Cycle 4	0.072 ± 0.003	14.0 ± 0.7	0.579 ± 0.326	-0.180 ± 0.020
Cycle 5	0.079 ± 0.014	13.8 ± 2.0	0.478 ± 0.315	-0.197 ± 0.019
2.5 g/L CSL
Cycle 1	0.079 ± 0.003	14.4 ± 1.0	0.831 ± 0.158	-0.214 ± 0.024
Cycle 2	0.084 ± 0.006	13.3 ± 0.6	0.841 ± 0.056	-0.208 ± 0.012
Cycle 3	0.094 ± 0.001	12.0 ± 0.0	0.728 ± 0.070	-0.196 ± 0.005
Cycle 4	0.089 ± 0.003	12.5 ± 0.4	0.718 ± 0.009	-0.189 ± 0.002
Cycle 5	0.076 ± 0.001	14.0 ± 0.4	0.688 ± 0.162	-0.210 ± 0.015

⁺Specific xylose consumption rate was calculated by dividing the xylose consumed by the time period and average viable dry cell weight concentration as correlated from capacitance readings;
calculated from ^a0 to 24 h, ^b2 to 4.5 h, or ^c8 to 24 h;
average cell viable cell concentration was calculated using the integral method.

ISSN: 2731-3654