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Table 2 Enzymatic hydrolysis of flowthrough pretreated whole slurries

From: Enhancement of total sugar and lignin yields through dissolution of poplar wood by hot water and dilute acid flowthrough pretreatment

Pretreatment conditionsa

Enzyme loadingb

1% (w/w) Bovine serum albumin

Enzymatic glucose yield (%)

Enzymatic xylose yield (%)

t1

t2

t3

t4

t5

t6

t1

t2

t3

t4

t5

t6

A

Low

Yes

45.9

48.7

51.5

51.5

53.6

54.9

43.7

79.3

88.0

89.8

90.2

91.3

A

Medium

Yes

56.0

58.5

63.6

66.3

70.8

73.0

62.8

88.1

92.8

95.0

96.0

97.8

A

High

Yes

81.6

93.1

100

100

100

100

94.9

96.8

97.0

97.8

98.0

98.3

A

Low

No

41.5

42.8

43.0

44.9

47.2

49.1

38.6

74.0

84.9

89.2

90.0

90.8

A

Medium

No

51.5

53.8

56.5

59.9

62.5

66.4

58.1

85.0

88.1

92.2

95.0

96.1

A

High

No

73.4

86.5

92.7

95.6

98.1

99.0

89.7

94.1

95.7

96.0

97.1

97.8

B

Low

Yes

57.1

72.6

75.4

77.4

77.6

79.6

48.9

82.5

90.3

91.8

92.1

92.8

B

Medium

Yes

67.3

83.1

88.3

92.8

94.7

97.7

68.3

90.6

91.0

93.2

95.5

97.1

B

High

Yes

99.5

99.8

100

100

100

100

93.7

96.7

97.8

98.1

98.2

98.8

B

Low

No

52.7

65.6

66.2

69.8

71.3

73.3

43.9

77.8

86.3

91.0

91.7

92.2

B

Medium

No

62.9

77.9

82.9

86.5

90.3

93.3

63.8

87.6

89.8

93.0

94.9

97.0

B

High

No

91.2

94.4

97.8

100

100

100

89.0

96.2

96.9

97.2

98.0

98.6

  1. aA: 270°C, 10 minutes, 25 mL/minute, water only; B: 240°C, 8 minutes, 25 mL/minute, 0.05% (w/w) H2SO4. bLow enzyme loading: 3 mg protein Ctec2 (2.8 filter paper units (FPU)) with 0.6 mg protein Htec2/g glucan + xylan; medium enzyme loading: 10 mg protein Ctec 2 (9.3 FPU) with 2 mg Htec2/g glucan + xylan; high enzyme loading: 100 mg protein Ctec 2 (93 FPU) with 20 mg Htec2/g glucan + xylan.
  2. Enzymatic hydrolysis time-t1: 4 hrs; t2: 24 hrs; t3: 48 hrs; t4: 72 hrs; t5: 96 hrs; t6: 120 hrs.