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Table 2 Enzymatic hydrolysis of flowthrough pretreated whole slurries

From: Enhancement of total sugar and lignin yields through dissolution of poplar wood by hot water and dilute acid flowthrough pretreatment

Pretreatment conditionsa Enzyme loadingb 1% (w/w) Bovine serum albumin Enzymatic glucose yield (%) Enzymatic xylose yield (%)
t1 t2 t3 t4 t5 t6 t1 t2 t3 t4 t5 t6
A Low Yes 45.9 48.7 51.5 51.5 53.6 54.9 43.7 79.3 88.0 89.8 90.2 91.3
A Medium Yes 56.0 58.5 63.6 66.3 70.8 73.0 62.8 88.1 92.8 95.0 96.0 97.8
A High Yes 81.6 93.1 100 100 100 100 94.9 96.8 97.0 97.8 98.0 98.3
A Low No 41.5 42.8 43.0 44.9 47.2 49.1 38.6 74.0 84.9 89.2 90.0 90.8
A Medium No 51.5 53.8 56.5 59.9 62.5 66.4 58.1 85.0 88.1 92.2 95.0 96.1
A High No 73.4 86.5 92.7 95.6 98.1 99.0 89.7 94.1 95.7 96.0 97.1 97.8
B Low Yes 57.1 72.6 75.4 77.4 77.6 79.6 48.9 82.5 90.3 91.8 92.1 92.8
B Medium Yes 67.3 83.1 88.3 92.8 94.7 97.7 68.3 90.6 91.0 93.2 95.5 97.1
B High Yes 99.5 99.8 100 100 100 100 93.7 96.7 97.8 98.1 98.2 98.8
B Low No 52.7 65.6 66.2 69.8 71.3 73.3 43.9 77.8 86.3 91.0 91.7 92.2
B Medium No 62.9 77.9 82.9 86.5 90.3 93.3 63.8 87.6 89.8 93.0 94.9 97.0
B High No 91.2 94.4 97.8 100 100 100 89.0 96.2 96.9 97.2 98.0 98.6
  1. aA: 270°C, 10 minutes, 25 mL/minute, water only; B: 240°C, 8 minutes, 25 mL/minute, 0.05% (w/w) H2SO4. bLow enzyme loading: 3 mg protein Ctec2 (2.8 filter paper units (FPU)) with 0.6 mg protein Htec2/g glucan + xylan; medium enzyme loading: 10 mg protein Ctec 2 (9.3 FPU) with 2 mg Htec2/g glucan + xylan; high enzyme loading: 100 mg protein Ctec 2 (93 FPU) with 20 mg Htec2/g glucan + xylan.
  2. Enzymatic hydrolysis time-t1: 4 hrs; t2: 24 hrs; t3: 48 hrs; t4: 72 hrs; t5: 96 hrs; t6: 120 hrs.