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Table 3 Material balance a of flowthrough pretreatment (stage 1) and enzymatic hydrolysis (stage 2)

From: Enhancement of total sugar and lignin yields through dissolution of poplar wood by hot water and dilute acid flowthrough pretreatment

Pretreatment conditions Enzyme loadingb Stage 1 Stage 1 + stage 2
Soluble fractions Insoluble fractions Soluble fractions Insoluble fractions
Glu (g) GOS(g) Xyl (g) XOS (g) SL (g) L-GOS (g) ISL (g) Glu (g) Xyl (g) SL (g) ISL (g)
A High 7.5 19.7 4.0 14.7 1.2 23.8 21.8 52.7 18.6 1.2 21.8
B Medium 31.2 15.5 6.8 12.2 0.5 4.9 23.0 50.8 18.7 0.5 23.0
  1. aThe calculation is based on 100 g dry weight poplar wood, which contains 54.2 g glucose, 19.1 g xylose and 23.7 g lignin; A: 270°C, water only, 25 mL/minute, 10 minutes; B: 240°C, 0.05% (w/w) H2SO4, 25 mL/minute, 8 minutes; the water consumption for water only (that is, 270°C, 10 minutes, 25 mL/minute) and dilute acid (that is, 240°C, 0.05% (w/w) H2SO4, 8 minutes, 25 mL/minute) operations was approximately 250 mL and 200 mL, respectively, which led to around 0.2 to 0.25% (w/w) overall solid to liquid ratio with a solid loading of 0.5 g. bHigh enzyme loading: 100 mg protein Ctec 2 (93 filter paper units (FPU)) with 20 mg Htec2/g glucan + xylan; medium enzyme loading: 10 mg protein Ctec 2 (9.3 FPU) with 2 mg Htec2/g glucan + xylan. Glu, glucose; Xyl, xylose; XOS, xylooligomers; GOS, soluble glucose oligomers; L-GOS, insoluble glucan derivatives; SL, soluble lignin; ISL, insoluble lignin.