Figure 3
Kinetic parameters of the β-glucosidase (BGL)-displaying strains. After 48 h cultivation, BGL activity of BY-BG-SA and SS cells was measured between 0.02 and 0.5 mM p-nitrophenyl-β-D-glucopyranoside (p NPG) at pH 5.0 and 30°C. K m and Vmax values were calculated by weighted non-linear least-squares analysis of the raw data [23]. SA, SED1 promoter and SAG1 anchoring region; SS, SED1 promoter and SED1 anchoring region.