The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons

Table 1 Cellulose hydrolysis activities of C. thermocellum strains

C. thermocellum strains	V_max(mM glucose equivalent per h/%)	t_max(h/fold)
WT (DSM1313)^*	2.84/100	26/1.00
CipA-ΔXDocII	2.58/91	41/1.58
CipA-Δ6CohI	1.54/54	81/3.16
CipA-ΔCBM-1^†	0.31/11	115/4.42
CipA-ΔCBM-2^†	0.22/8	206/7.92
ΔSdbA	2.44/86	26/1.00
ΔOrf2p	2.31/81	28/1.08
ΔOlpB	2.19/77	40/1.54
Δ7CohII	2.14/75	36/1.38

^*V_max and t_max of the WT strain are set as 100% and one fold, respectively. The curve-fitting parameters are listed in Additional file 10.
^†The targetron in CipA-ΔCBM-2 inserts at the very end of the second CohI module of CipA, possibly affecting the function of this module and resulting in lower V_max and longer t_max values compared with CipA-ΔCBM-1.

ISSN: 2731-3654