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Table 1 GC/MS data for sterol profiling from N. oceanica treated with sterol biosynthetic inhibitors

From: Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica

Sterols Structure RT (min) RRTc Sterol profile (%)
Control CLO TBF TEB TDM 25-AZA
Squalene 1 10.25 0.755 -* - 12.0 - - -
Cholesterol 2 13.58 1.000 74.0 70.4 61.6 62.0 56.2 81.3
Cholest-8-enol 3 13.75 1.012 - - - - 8.2 -
Desmosterol 4 13.91 1.024 - - - - - 3.0
Pollinastanol 5 14.33 1.056 - - - - 13.1 -
4, 14-Dimethylcholest-8-enol 6 14.53 1.070 - - - 1.7 - -
24-Methylenecholesterol 7 14.59 1.074 1.4 2.5 0.7 2.7 - 4.0
24(25)-Dihydrolanosterol 8 15.18 1.117 - - - 1.7 - -
4-Methylpollinasterol 9 15.47 1.139 - - - - 3.7 -
24-Methylcholesta-5, 25(27)-dienol 10 15.51 1.142 0.9 1.0 0.9 0.8 0.3 1.0
Fucosterol 11 15.68 1.155 10.1 10.1 10.3 7.2 6.8 6.0
Obtusifoliol 12 15.73 1.158 - - - 0.2 - -
Isofucosterol 13 15.91 1.171 13.5 16.1 14.3 8.9 9.3 4.8
Stigmasta-8, 24(28) E-dienol 14 15.93 1.173 - - - - 0.4 -
Cycloartenol 15 16.06 1.182 - - - 12.8 - -
Stigmasta-8, 24(28) Z-dienol 16 16.16 1.190 - - - - 1.9 -
Cycloartanol 17 16.59 1.222 - - - 2.1 - -
  1. Cultures in exponential phase were diluted in fresh media and grown for 12 h under 50 ╬╝mol photons m-2 s-1 light. Subsequently, cultures were treated with inhibitors at a concentration of IC50 or an equivalent amount of DMSO (not exceeding a final concentration of 0.1%). Cell aliquots were collected for sterol profiling four days later. Three biological replicates of algal cultures were established under each treatment. Abbreviations: RRTc, relative retention time to cholesterol; CLO, clomazone; TBF, terbinafine; TEB, tebuconazole; TDM, tridemorph; 25-AZA, 25-azalanosterol. The asterisk (*) indicates a value below the detection limit under current experimental conditions. The structure numbers in boldface are listed in Additional file 2: Figure S3.