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Table 1 GC/MS data for sterol profiling from N. oceanica treated with sterol biosynthetic inhibitors

From: Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica

Sterols

Structure

RT (min)

RRTc

Sterol profile (%)

Control

CLO

TBF

TEB

TDM

25-AZA

Squalene

1

10.25

0.755

-*

-

12.0

-

-

-

Cholesterol

2

13.58

1.000

74.0

70.4

61.6

62.0

56.2

81.3

Cholest-8-enol

3

13.75

1.012

-

-

-

-

8.2

-

Desmosterol

4

13.91

1.024

-

-

-

-

-

3.0

Pollinastanol

5

14.33

1.056

-

-

-

-

13.1

-

4, 14-Dimethylcholest-8-enol

6

14.53

1.070

-

-

-

1.7

-

-

24-Methylenecholesterol

7

14.59

1.074

1.4

2.5

0.7

2.7

-

4.0

24(25)-Dihydrolanosterol

8

15.18

1.117

-

-

-

1.7

-

-

4-Methylpollinasterol

9

15.47

1.139

-

-

-

-

3.7

-

24-Methylcholesta-5, 25(27)-dienol

10

15.51

1.142

0.9

1.0

0.9

0.8

0.3

1.0

Fucosterol

11

15.68

1.155

10.1

10.1

10.3

7.2

6.8

6.0

Obtusifoliol

12

15.73

1.158

-

-

-

0.2

-

-

Isofucosterol

13

15.91

1.171

13.5

16.1

14.3

8.9

9.3

4.8

Stigmasta-8, 24(28) E-dienol

14

15.93

1.173

-

-

-

-

0.4

-

Cycloartenol

15

16.06

1.182

-

-

-

12.8

-

-

Stigmasta-8, 24(28) Z-dienol

16

16.16

1.190

-

-

-

-

1.9

-

Cycloartanol

17

16.59

1.222

-

-

-

2.1

-

-

  1. Cultures in exponential phase were diluted in fresh media and grown for 12 h under 50 ╬╝mol photons m-2 s-1 light. Subsequently, cultures were treated with inhibitors at a concentration of IC50 or an equivalent amount of DMSO (not exceeding a final concentration of 0.1%). Cell aliquots were collected for sterol profiling four days later. Three biological replicates of algal cultures were established under each treatment. Abbreviations: RRTc, relative retention time to cholesterol; CLO, clomazone; TBF, terbinafine; TEB, tebuconazole; TDM, tridemorph; 25-AZA, 25-azalanosterol. The asterisk (*) indicates a value below the detection limit under current experimental conditions. The structure numbers in boldface are listed in Additional file 2: Figure S3.