Table 9 Plan of the experiments for screening the nine Chlorella strains in the laboratory
Trial | Duration (days) | Culture system | Culture regime | Light regime and intensity | Culture medium | Temperature |
|---|---|---|---|---|---|---|
1. Batch growth | 7 | 300 mL bubble tubes | Batch | Continuous light (400 μmol photons m−2 s−1) | Isolationa medium | 25°C |
2. Semi-continuous growth | 7 | 300 mL bubble tubes | 30% daily dilution | Continuous light (400 μmol photons m−2 s−1) | Isolationa medium | 25°C |
3. Starvation | 7 | 300 mL bubble tubes | Batch | Continuous light (400 μmol photons m−2 s−1) | Isolationa medium | 25°C |
4. High temperature | 4 | 40 mL bubble tubes | Batch | Light:dark cycle (8 h:16 h) (200 μmol photons m−2 s−1) | Isolationa medium | 40°C for 8 h - 25°C for 16 h; control cultures 24 h at 25°C |
5. Culture medium | 7 | 40 mL bubble tubes | Batch | Continuous light (200 μmol photons m−2 s−1) | BG11 and F | 25°C |