Table 1 Strains and plasmids used in this study
From: Overcoming inefficient cellobiose fermentation by cellobiose phosphorylase in the presence of xylose
Strains/plasmids | Characteristics | Reference |
|---|---|---|
D452-2 | MATalpha, leu2, his3, ura3, and can1 | [19] |
SR8 | ald6Δ of the evolved strain D452-2 leu2::LEU2 P TDH3 -XYL1-T TDH3 ; ura3::URA3 P TDH3 -XYL1- T TDH3 P PGK1 -XYL2- T PGK1 P TDH3 -XYL3- T TDH3 ; his1::HIS1 P PGK1 -XYL2- T PGK1 P TDH3 -XYL3- T TDH3 | [20] |
SR8-a | SR8 ura3 | Courtesy of Dr. Soo Rin Kim |
pCT | pRS426- P PGK1 -cdt-1(F213L)-eGFP-t CYC1 | [10] |
pSd | pRS425- P PGK1 -SdCBP-t CYC1 | [10] |
pCS | pRS426-P PGK1 -cdt-1(F213L)-eGFP-t CYC1 -P PGK1 -SdCBP-t CYC1 | This study |
pET-Sd | pET302-NT/6his-SdCBP | This study |