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Table 1 Rate of reaction of free and immobilised enzyme at different substrate concentrations using Michaelis-Menten kinetics

From: Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass

 

Free enzyme

Immobilised enzyme

V max (mg/mL/min)

0.72 ± 0.1

2.0 ± 0.6

K m (mg/mL)

0.87 ± 0.3

2.6 ± 1.3

  1. 95% confidence level (GraphPad Prism 6).