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Table 5 Performance based on biomass concentration, lipid accumulation, and CO 2 fixation of salinity-gradient culturing of Chlamydomonas sp. JSC4 with stepwise addition of sea salt at different increments

From: Optimizing biodiesel production in marine Chlamydomonassp. JSC4 through metabolic profiling and an innovative salinity-gradient strategy

Sea salt addition (% d-1)

Cultivation time under nitrogen depletion (days)

Biomass concentration (g L-1)

Lipid content (%)

Lipid productivity (mg L-1d-1)

CO2fixation rate (mg L-1d-1)

0.0 (batch)

0

1.426 ± 0.008

15.8 ± 0.1

111 ± 0

1319 ± 6

3

2.232 ± 0.007

41.9 ± 1.8

159 ± 8

731 ± 3

5

2.467 ± 0.064

49.8 ± 2.3

152 ± 11

561 ± 16

0.5

0

1.471 ± 0.010

15.3 ± 0.3

101 ± 3

1243 ± 9

3

2.129 ± 0.004

59.4 ± 0.7

223 ± 2

706 ± 1

5

2.180 ± 0.042

63.7 ± 0.8

175 ± 3

515 ± 3

1.0

0

1.445 ± 0.021

15.7 ± 0.6

102 ± 5

1219 ± 19

3

2.044 ± 0.015

60.2 ± 2.5

217 ± 7

677 ± 5

5

2.082 ± 0.079

64.9 ± 2.7

170 ± 14

491 ± 20

1.5

0

1.436 ± 0.007

15.5 ± 0.6

100 ± 3

1211 ± 6

3

2.009 ± 0.001

53.7 ± 2.0

190 ± 7

665 ± 0

 

5

2.044 ± 0.090

59.4 ± 0.3

152 ± 6

482 ± 22

  1. The values are the mean ± standard deviation of three replicated experiments. (Light intensity = 200 μmol m-2 s-1; CO2 aeration = 2.0%; CO2 flow rate = 0.05 vvm).