Figure 4

Analysis of flux control over lactic acid formation. Double logarithmic plot relating relative LDH enzyme activity (abscissa) with relative flux towards the final product lactic acid (ordinate). Mutant strains that contain LDH as the only exogenous gene (black squares) were used to generate the fit (black solid line) and were used to calculate control coefficients. Data for the PK co-expressing strains with low amounts of LDH expression, SYW003 and SYW004, are shown with green diamonds. SAW041, the strain with PK co-expression with high amounts of LDH expression, is shown with a red diamond. Data for mutant strains SAA005 and SAA025 were taken from [14]. Error bars represent the SD of biological replicates (n = 3), except for SAW001, SYW003, and SYW004 where n = 2; if error bars are not visible, they are smaller than the data point symbol.