Figure 5From: Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803Growth and lactic acid production in Synechocystis mutant strains expressing alternative LDH enzymes. (A, B) Growth curves and (volumetric) rate of production (left chart); L-lactic acid yield and partitioning (right chart) for (A) SAV001, expressing the Ll LDHL39R and (B) SAA029, expressing the Cg LDHWT, and (C) Enzymatic activity of cell-free extracts of SAV001 towards NADH and NADPH, relative to the NADH-dependent activity of SAA023. Error bars represent the SD of biological replicates (n = 3); if error bars are not visible, they are smaller than the data point symbol. (D) CBB-stained gel with corresponding cell-free extracts, indicating the overexpression of the unmodified and Ll LDHL39R in SAA023 and SAV001, respectively (*), and the Cg LDHWT in SAA029 (<). The solid vertical line indicates a composite gel. Lanes are aligned according to the pattern of the wild-type proteins visible in the cell-free extract. The stippled vertical line separates wild type from the mutant strains’ cell-free extracts.Back to article page