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Figure 5 | Biotechnology for Biofuels

Figure 5

From: Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803

Figure 5

Growth and lactic acid production in Synechocystis mutant strains expressing alternative LDH enzymes. (A, B) Growth curves and (volumetric) rate of production (left chart); L-lactic acid yield and partitioning (right chart) for (A) SAV001, expressing the Ll LDHL39R and (B) SAA029, expressing the Cg LDHWT, and (C) Enzymatic activity of cell-free extracts of SAV001 towards NADH and NADPH, relative to the NADH-dependent activity of SAA023. Error bars represent the SD of biological replicates (n = 3); if error bars are not visible, they are smaller than the data point symbol. (D) CBB-stained gel with corresponding cell-free extracts, indicating the overexpression of the unmodified and Ll LDHL39R in SAA023 and SAV001, respectively (*), and the Cg LDHWT in SAA029 (<). The solid vertical line indicates a composite gel. Lanes are aligned according to the pattern of the wild-type proteins visible in the cell-free extract. The stippled vertical line separates wild type from the mutant strains’ cell-free extracts.

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