Figure 6
Growth and lactic acid production of Synechocystis strains overexpressing the L-LDH WT and the L-LDH V38R of B. subtilis . (A) Growth curves and (volumetric) lactic acid rates over time of SAA015 (expressing the non-codon optimized ldh), SAA027 (expressing a codon optimized ldh), and SAA028 (expressing the V38R-mutated version of the codon optimized ldh of B. subtilis). (B) Fixed carbon partitioned over biomass and the product lactic acid, at the subsequent respective time intervals (left ordinate). Lactic acid production yields (filled triangles) are indicated on the right ordinate. (C) CBB-stained gel with corresponding cell-free extracts and the His-tagged purified proteins. (D) Enzymatic activity of cell-free extracts of SAA015, SAA027, and SAA028. (E) Enzymatic activity of the purified enzymes, B. subtilis LDHWT and LDHV38R. Error bars represent the SD of biological replicates (n = 3).