Table 1 Strains used in this study

Wild type

Wild-type Synechocystis

D. Bhaya (Stanford)

SAA005

PpsbA2::psbA2:: _Ll ldh _na

[14]

SAA024

PrnpB:: _Ll ldh _na

[14]

SAA025

Ptrc1:: _Ll ldh _na

[14]; Ptrc1 is one trc promoter with one operator sequence [18]; native version of L. lactis ldh

SAA023

Ptrc1:: _Ll ldh _co

[14]; codon optimized version of L. lactis ldh

SAW001

Ptrc2:: _Ll ldh _co

Ptrc2 is one trc promoter flanked by the operator sequence [18]

SAA026

Ptrc1:: _Ll ldh _co ::ldh _na

Double ldh gene dosage on genome

SAW035

pDF_LDH

Expression from plasmid

SAW039

Ptrc1:: _Ll ldh _co , pDF_LDH

Combined expression from genome and plasmid

SYW001

Ptrc2:: _Syn pk _co :: Ptrc2:: _Ll ldh _co

pk from Synechocystis

SYW003

Ptrc2:: _Ef pk _co :: Ptrc2:: _Ll ldh _co

pk from E. faecalis

SYW004

Ptrc2:: _Ec pk _co :: Ptrc2:: _Ll ldh _co

pk from E. coli

SYW005

Ptrc2:: _Ll pk _co :: Ptrc2:: _Ll ldh _co

pk from L. lactis

SAW041

Ptrc2:: _Ef pk _co :: Ptrc2:: _Ll ldh _co , pDF_LDH

SYW003 additionally carrying pDF_LDH

SAW033

Ptrc1:: _Ll ldh _co , partialΔ::ppc

Partially (stable) segregated strain of the ppc knockout attempt

SAA015

Ptrc1:: _Bs ldh _na

[10]; native version of ldh from B. subtilis

SAA027

Ptrc1:: _Bs ldh _co WT

Codon optimized version of ldh from B. subtilis

SAA028

Ptrc1:: _Bs ldh _co V38R

V38R mutant of the codon optimized ldh from B. subtilis

SAA029

Ptrc1:: _Cg ldh _co WT

Codon optimized ldh from C. gunnari

SAA030

Ptrc1:: _Cg ldh _co V53R, E232M

V53R and E232M mutant of the codon optimized ldh from C. gunnari

SAV001

Ptrc1:: _Ll ldh _co L39R

L39R mutant of the codon optimized ldh from L. lactis

SAA035

Ptrc1:: _Po ldh _co I29R

I29R mutant of the codon optimized ldh from P. ovale