Systematic and evolutionary engineering of a xylose isomerase-based pathway in Saccharomyces cerevisiae for efficient conversion yields

Table 2 Comparison of representative previously reported xylose fermentation performances

Strains	Strain description	Conditions	Xylose consumption rate (g/g⁻¹ h⁻¹)	Ethanol production rate (g/g⁻¹ h⁻¹)	Ethanol yield (g/g⁻¹)	Reference
SXA-R2P-E	xylA3, tal1, XKS1, Δgre3, Δpho13* Evolved (24 days)	Anaerobic batch in bioreactor, synthetic medium (40 g/L xylose)	0.98	0.44	0.45	This study
H131-A3-CS	xylA, xyl3, TAL1, TKL1, RPE1, RKI1, evolved (160 days)	Anaerobic batch in bioreactor, syntheticmedium (40 g/L xylose)	0.94	0.40	0.43	[16]
H131-A3-ALCS	xylA, xyl3, TAL1, TKL1, RPE1, RKI1, evolved (160 days)	Anaerobic batch in bioreactor, synthetic medium (40 g/L xylose)	1.87	0. 77	0.41	[16]
CEN.PK- RWB218	xylA, XKS1, TKL1, RPE1, RKI1, Δgre3, evolved (70 days)	Anaerobic batch in bioreactor, synthetic medium (20 g/L xylose)	-	0.49 (projected)	0.41	[17]
SR8	Xyl1, xyl2, xyl3, Δald6, Evolved (no detailed information)	Anaerobic batch in flasks, synthetic medium (40 g/L xylose)	0.65	0.25	0.39	[24]
CEN.PK2-1C -TMB 3424	Xyl1, xyl2, XKS1, GAL2, TKL1, RPE1, RKI1, Δgre3 Selected (70 days)	Anaerobic batch in bioreactor, synthetic medium (60 g/L xylose)	0.89	0.32	0.36	[6]
SR8	Xyl1, xyl2, xyl3, Δald6, Evolved (no detailed information)	Oxygen-limited batch in flasks, complete medium (40 g/L xylose)	0.87	0.28	0.31	[15]

Fermentation performance of S. cerevisiae SXA-R2P-E was compared with previously reported results for representative isomerase and oxidoreductase pathways.

ISSN: 2731-3654