Figure 5

Validation of RNA-seq by qRT-PCR and an enzyme activity assay. (A) Gene expression detected by qRT-PCR in Chlorella ellipsoidea. The relative expression of 20 upregulated and two downregulated genes related to lipid and fatty acid metabolism was determined by qRT-PCR. The data represent the means ± SD of three replicate experiments and were analyzed by Student’s t-test (n = 3). *P <0.05; **P <0.01. (B) ACCase activity in crude cell extracts of CK and transgenic GmDof4 strains. The data represent the means ± SD of three replicate experiments and were analyzed by Student’s t-test (n = 3). *P <0.05. ACCase, acetyl-coenzyme A carboxylase; CK, pCK transgenic strains.