Figure 5

Improved production of reducing sugars from milled Jerusalem artichoke stalks by synergy of Ch2-2 enzymes and commercial cellulase or xylanase. A. Hydrolysis by binary (commercial cellulases/xylanase, commercial cellulases/Ch2-2 enzymes and commercial xylanase/Ch2-2 enzymes) or ternary mixtures (commercial cellulases/xylanase/Ch2-2 enzymes) at 15°C for 24 hours and then 50°C for 96 hours. B. Hydrolysis by binary or ternary mixtures at 28°C for 120 hours. C. Hydrolysis by binary or ternary mixtures at 15°C for 120 hours. Substrate control: milled Jerusalem artichoke stalks without enzymes. Enzyme control: reactions with each enzyme (commercial cellulases, commercial xylanase or Ch2-2 enzymes) alone.