SDS-PAGE analysis of corn stover leaf extracts. Transgenic corn stover leaf extracts prepared at 16:1 (lane 2), 12:1 (lane 3), and 8:1 (lane 4) buffer-to-dry mass ratios were resolved using NuPAGE® 4-16% Bis-Tris gel (Invitrogen) with MES SDS buffer. Extracts from non-transgenic corn stover leaf prepared under corresponding conditions (lanes 5-7) were resolved as negative controls. SeeBlue® Plus2 (Invitrogen) was used as the size standard (lane 1). Equal volumes (10 μL) of extracts were analysed.