Figure 3
Saccharification of H 2 SO 4 steam exploded bagasse by mixtures of Celluclast 1.5 L and corn stover leaf extracts. Celluclast 1.5 L at dosages of 4 (circles), 6 (triangles), 10 (squares), and 20 (diamonds) FPU/g glucan were supplemented with MULase activity from transgenic corn stover leaf extracts containing recombinant CBH (black symbols) and used to saccharify H2SO4 steam exploded bagasse for 24 h. The numeral 1on the x-axis represents the total MULase activity present in the indicated dosage of Celluclast 1.5 L. Values above 1 indicate the addition of corn stover leaf extract containing recombinant CBH to Celluclast 1.5 L in units of MULase activity equal to the total MULase activity in Celluclast 1.5 L at each of the indicated dosages. Each reaction was supplemented with 50 μg β-glucosidase/g glucan. Equal volumes of extracts from non-transgenic corn stover leaves prepared under the same conditions were assessed for comparison (open symbols). Glucose release from cellulose was monitored using a colorimetric (GOPOD Format) assay and the results reported as the percentage of glucan converted to glucose. All samples were analysed in triplicate, and error bars represent standard deviation.