Figure 5

Saccharification of pretreated bagasse by transgenic corn stover leaf extracts containing recombinant CBH. (a) Pretreated bagasse samples were saccharified for 24 h with extracts from transgenic corn stover leaf containing recombinant CBH. The numerals on the x-axis represent the total loading of MULase activity from transgenic corn stover leaf extract, in multiples of the total MULase activity present in Cellic CTec2 at a dosage of 2 FPU/g glucan. The transgenic-extract MULase loadings designated as “1 + 4” in this figure are each thus equal to the loadings of “additional” transgenic-extract MULase activity added to Cellic CTec2 in the loading designated as “1 + 5“ in Figure 4. Each reaction was supplemented with 50 μg β-glucosidase/g glucan. Glucose release from glucan was monitored using a colorimetric (GOPOD) assay and the results reported as the percentage of glucan converted to glucose. All samples were analysed in triplicate, and error bars represent standard deviation. (b) Comparison between the ability of corn stover leaf extract containing recombinant CBH to hydrolyse pretreated bagasse and its ability to enhance the performance of a commercial cellulase mixture. The black line indicates equal performance alone and in combination with a commercial cellulase mixture.