Figure 6
Saccharification of pretreated bagasse with mixtures of Cellic CTec2 and transgenic corn stover leaf extract. (a) H2SO4 steam explosion. (b) NaOH steam explosion. (c) Glycerol/HCl steam explosion. (d) EC/EG. Pretreated bagasse samples were saccharified with a dosage of Cellic CTec2 that resulted in about 30% glucan conversion after 24 h. Cellic CTec2 at these dosages was supplemented with MULase activity from transgenic corn stover leaf extract containing recombinant CBH (black symbols). Values above 1 indicate the addition of corn stover leaf extract containing recombinant CBH to Cellic CTec2 in units of MULase activity equal to the total MULase activity in Cellic CTec2 at each of the indicated dosages. Equal volumes of extracts from non-transgenic corn stover leaves prepared under the same conditions were assessed for comparison (open symbols).