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Figure 2 | Biotechnology for Biofuels

Figure 2

From: Heterologous complementation of a pyrF deletion in Caldicellulosiruptor hydrothermalisgenerates a new host for the analysis of biomass deconstruction

Figure 2

Plasmid pJGW07 isolated directly from C. hydrothermalis Δ pyrF . (A) pJGW07 constructed by replacing the C. bescii pyrF gene with the C. thermocellum ATCC 4705 homolog Cthe0951. The hatched region was derived from C. bescii native plasmid pBAS2. AprR, apramycin resistance casette; repA, replication initiator for pSC101 replication origin; par, partitioning locus. (B) Agarose gel depicting pJGW07 plasmid DNA extracted from different sources. M, molecular weight standards; E, pJGW07 purified from E. coli; C, pJGW07 purified from C. hydrothermalis.

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