Skip to main content

Table 1 Strains and plasmids used in this study

From: Heterologous complementation of a pyrF deletion in Caldicellulosiruptor hydrothermalisgenerates a new host for the analysis of biomass deconstruction

Strain or plasmid Genotype/phenotype
JWCH001 C. hydrothermalis DSM 18901 wild -type (ura+/5-FOAS)
JWCH006 C. hydrothermalis ΔpyrF (ura-/5-FOAR)
JWCH008 C. hydrothermalis ΔpyrF ΔchyI (ura-/5-FOAR)
JWCH009 C. hydrothermalis ΔpyrF harboring pJGW07 (ura+/5-FOAS)
JW401 DH5α containing pJGW03 (ApramycinR)
JW402 DH5α containing pJGW07 (ApramycinR)
pDCW89 E. coli/Caldicellulosiruptor shuttle vector (C. bescii pyrF)
pDCW88 Caldicellulosiruptor non-replicating vector (C. bescii pyrF)
pDCW151 Caldicellulosiruptor chyI deletion vector (C. bescii pyrF)
pJGW03 Caldicellulosiruptor chyI deletion vector (C. thermocellum pyrF)
pJGW07 E. coli/Caldicellulosiruptor shuttle vector (C. thermocellum pyrF)