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Table 2 Transformation efficiency (CFU/μg DNA)

From: Heterologous complementation of a pyrF deletion in Caldicellulosiruptor hydrothermalisgenerates a new host for the analysis of biomass deconstruction

Strain

pJGW07

pJGW07M

pDCW89

pDCW89M

JWCH006 Δ pyrF

33 ± 24

22 ± 10

18 ± 20

37 ± 43

JWCH008 Δ pyrF Δ chyI

395 ± 301

241 ± 174

58 ± 48

100 ± 90

  1. The suffix M denotes plasmid methylated with C. bescii M.CbeI methyltransferase.
  2. Results represent the average ± standard deviation of three biologically independent transformation experiments.