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Table 4 Sterol composition, dry weight biomass and squalene titre of YUG37- ERG1 cultured using GJ

From: Co-production of ethanol and squalene using a Saccharomyces cerevisiae ERG1 (squalene epoxidase) mutant and agro-industrial feedstock

DOX Sterol composition (mg sterol g-1) Total Biomass Squalene titre
(μg mL-1) a14α-DM b14α-M Ergosterol Squalene (mg g-1) (g L-1) (mg L-1)
0 1.09 [0.23] 0.47 [0.16] 3.51 [0.83] 0.19 [0.09] 5.25 [0.35] 4.80 [0.28] 0.88 [0.39]
0.025 0.97 [0.46] 0.27 [0.27] 2.64 [0.40] 3.98 [0.68] 7.85 [0.35] 4.50 [0.28] 18.0 [4.18]
0.05 0.73 [0.21] 0.28 [0.17] 2.00 [0.34] 5.14 [0.25] 8.15 [0.21] 2.25 [0.21] 11.5 [0.53]
0.5 0.31 [0.07] 0.09 [0.01] 2.63 [0.48] 5.92 [0.27] 8.95 [0.15] 1.78 [0.11] 10.5 [1.11]
5 0.10 [0.01] 0.01 [0.01] 1.27 [0.15] 7.89 [0.25] 9.28 [0.11] 1.44 [0.04] 11.4 [0.70]
50 1.31 [0.46] 7.84 [0.25] 9.15 [0.21] 1.45 [0.07] 11.4 [0.20]
**5 4.34 [0.07] 2.22 [0.28] 6.55 [0.35] 5.40 [0.14] 12.0 [1.83]
**50 3.80 [0.37] 2.45 [0.02] 6.25 [0.35] 5.20 [0.14] 12.7 [0.24]
  1. All cultures maintained at 20°C in the Bioscreen. Mean values (n = 3 [±SD]); DOX = doxycycline. Maximum squalene content and titre are emboldened. Asterisks indicate sequential production experiments supplemented with additional GJ + DOX after 48 h growth in the absence of DOX.
  2. Strikethrough = not detected.
  3. a = sum of all 14α-demethylated sterols; b = sum of 14α-methylated sterols.