Figure 5

Glycome profiling of untreated, uninoculated, and microbial incubated switchgrass biomass residues, shown for two biological replicates. Sequential cell wall extracts were prepared from switchgrass biomass samples using increasingly harsh reagents as explained in the Methods section. The ELISA response values are represented as heatmaps with bright yellow-red-dark blue scale indicating the intensities of the ELISA signals (bright yellow, red, and dark blue colors depict strong, medium, and no binding, respectively). The monoclonal antibodies are grouped based on the cell wall glycans they detect as depicted in the panel at the right-hand side of the heatmap. The gravimetric amounts of carbohydrate materials extracted from the walls by each extraction reagent are shown as bar graphs at the top of the figure.