Table 2 Comparison of various WS pretreatments with dry fractionation technology developed in this work
From: New dry technology of environmentally friendly biomass refinery: glucose yield and energy efficiency
Pretreatment conditions | Enzymatic hydrolysis conditions | Glucose (mg/g WS) | Reducing sugars (mg/g WS) | Solvent or water (L/Kg WS) | Chemical reagent (Kg/Kg WS) | Reference |
|---|---|---|---|---|---|---|
Mechanical | ||||||
50 - 600 μm | Celluclast-1.5 L Trichoderma reesei, 50°C, 20 hours | 150 | ND | 0 | 0 | [27] |
600 - 1000 μm | 120 | ND | 0 | 0 | ||
Ball milling 240 hour, 10 μm | Cellulase Trichoderma reesei, 37°C, 48 hours | N.D. | 270 | 0 | 0 | [28] |
Centrifugal milling: 270 μm | N.D. | 108 | 0 | 0 | ||
Milling + electrostatic fractionation: 50 -70 μm | Cellulase Trichoderma Longibrachiatum 37°C, 72 hours | 254* | 294* | 0 | 0 | This study |
Chemical | ||||||
750 μm, glycerol 70%, 230°C, 4 hours | Celluclast-1.5 L Trichoderma reesei, 50°C, 2.5 h | 268 | 423 | 20 | 0 | [30] |
750 μm, ionic liquid/WS ratio: 25/1 w/w, 150°C, 1 hour | 368 | 744 | 0.03 | 25 | ||
750 μm, 1% acid, 140°C, 40 minutes | 176 | 223 | 20 | 1 | ||
Physicochemical | ||||||
833 μm, water/WS ratio: 40/1, Steam explosion: 210°C, 10 minutes | Cellulase BTXL Trichoderma reesei, 50°C, 72 hours | ND | 117 | 40 | 0 | [31] |
833 μm, 50% v/v acetic acid, steam explosion: 220°C, 8 minutes | ND | 244 | 40 | 20 | ||
833 μm, 70% v/v ethanol steam explosion: 220°C, 5 minutes | ND | 264 | 40 | - | ||
833 μm, supercritical CO2 190°C, 12 mPa, 30 minutes | Cellulase Trichoderma reesei, 50°C, 72 hours | ND | 149 | ND | ND | [32] |
833 μm, steam explosion (A) 200°C, 15 minutes + supercritical CO2: 190°C, 12 mPa, 30 minutes | ND | 234 | ND | ND | ||