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Table 1 Strains/plasmid used in this study

From: Deletion of Caldicellulosiruptor besciiCelA reveals its crucial role in the deconstruction of lignocellulosic biomass

Strains/plasmid Genotype/phenotype Source
Caldicellulosiruptor bescii JWCB001 DSMZ6725 wild type (ura+/5-FOAS) DSMZa
C. bescii JWCB005 ΔpyrFA (ura-/5-FOAR) [23]
C. bescii JWCB018 ΔpyrFA ldh::ISCbe4 Δcbe1 (ura-/5-FOAR) [21],[22]
C. bescii JWCB029 ΔpyrFA ldh::ISCbe4 Δcbe1 ΔcelA (ura-/5-FOAR) This study
Escherichia coli JW330 DH5α containing pJFW52 (ApramycinR) This study
pJFW52 celA (Cbes1867) deletion vector (ApramycinR) This study
  1. aGerman Collection of Microorganisms and Cell cultures.