Table 1 Strains/plasmid used in this study

Caldicellulosiruptor bescii JWCB001

DSMZ6725 wild type (ura⁺/5-FOA^S)

DSMZ^a

C. bescii JWCB005

ΔpyrFA (ura^-/5-FOA^R)

[23]

C. bescii JWCB018

ΔpyrFA ldh::ISCbe4 Δcbe1 (ura^-/5-FOA^R)

[21],[22]

C. bescii JWCB029

ΔpyrFA ldh::ISCbe4 Δcbe1 ΔcelA (ura^-/5-FOA^R)

This study

Escherichia coli JW330

DH5α containing pJFW52 (Apramycin^R)

This study

pJFW52

celA (Cbes1867) deletion vector (Apramycin^R)

This study