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Table 5 Genes associated with core set of reactions under butanol stress conditions

From: Capturing the response of Clostridium acetobutylicumto chemical stressors using a regulated genome-scale metabolic model

  Stress level ORF Gene
Primary (K=1) Low CAC0316 Ornithine carbamoyltransferase (OCBT)
Med CAC0316 Ornithine carbamoyltransferase
High CAC0316 Ornithine carbamoyltransferase
CAC2390 N-acetyl-gamma-glutamyl-phosphate reductase (AGPR)
Secondary (K=2) Low CAC0974 Argininosuccinate lyase (ARGSL)
CAC2390 N-acetyl-gamma-glutamyl-phosphate reductase
Med CAC0974 Argininosuccinate lyase
CAC2390 N-acetyl-gamma-glutamyl-phosphate reductase
High CAC0974 Argininosuccinate lyase
CAC2389 Acetylglutamate kinase (ACGK)
Tertiary (K=3) Low CAC0973 Argininosuccinate synthase (ARGSS)
CAC2389 Acetylglutamate kinase
Med CAC0973 Argininosuccinate synthase
CAC2389 Acetylglutamate kinase
High CAC0973 Argininosuccinate synthase
Quaternary (K=4) [minimal effect on biomass] Low CAC2391 or CAC3020 Bifunctional ornithine acetyltransferase/N-acetylglutamate synthase (OACT/AGS)
Med CAC2391 or CAC3020 Bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
High CAC2388 or CAC0368 4-aminobutyrate aminotransferase acetylornithine/N-succinyldiaminopimelate aminotransferase (GABAT/ARGD)
  1. If regulatory bounds were imposed exclusively on the reactions in the core set, the flux ranges would match those of the model with all regulatory constraints. Regulation was removed from the primary core sets to obtain secondary cores sets, and so on. Biomass regulation reduced with increasing order of core sets.