Capturing the response of Clostridium acetobutylicumto chemical stressors using a regulated genome-scale metabolic model

Table 5 Genes associated with core set of reactions under butanol stress conditions

	Stress level	ORF	Gene
Primary (K=1)	Low	CAC0316	Ornithine carbamoyltransferase (OCBT)
	Med	CAC0316	Ornithine carbamoyltransferase
	High	CAC0316	Ornithine carbamoyltransferase
	High	CAC2390	N-acetyl-gamma-glutamyl-phosphate reductase (AGPR)
Secondary (K=2)	Low	CAC0974	Argininosuccinate lyase (ARGSL)
	Low	CAC2390	N-acetyl-gamma-glutamyl-phosphate reductase
	Med	CAC0974	Argininosuccinate lyase
	Med	CAC2390	N-acetyl-gamma-glutamyl-phosphate reductase
	High	CAC0974	Argininosuccinate lyase
	High	CAC2389	Acetylglutamate kinase (ACGK)
Tertiary (K=3)	Low	CAC0973	Argininosuccinate synthase (ARGSS)
	Low	CAC2389	Acetylglutamate kinase
	Med	CAC0973	Argininosuccinate synthase
	Med	CAC2389	Acetylglutamate kinase
	High	CAC0973	Argininosuccinate synthase
Quaternary (K=4) [minimal effect on biomass]	Low	CAC2391 or CAC3020	Bifunctional ornithine acetyltransferase/N-acetylglutamate synthase (OACT/AGS)
	Med	CAC2391 or CAC3020	Bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
	High	CAC2388 or CAC0368	4-aminobutyrate aminotransferase acetylornithine/N-succinyldiaminopimelate aminotransferase (GABAT/ARGD)

If regulatory bounds were imposed exclusively on the reactions in the core set, the flux ranges would match those of the model with all regulatory constraints. Regulation was removed from the primary core sets to obtain secondary cores sets, and so on. Biomass regulation reduced with increasing order of core sets.

ISSN: 2731-3654