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Table 6 Genes associated with core set of reactions under butyrate stress conditions

From: Capturing the response of Clostridium acetobutylicumto chemical stressors using a regulated genome-scale metabolic model

  Stress level ORF Gene
Primary (K=1) Low [minimal effect on biomass] CAC2113 Bifunctional pyrimidine regulatory protein PyrR/uracil phosphoribosyltransferase (UPRT)
Med [minimal effect on biomass] CAC0109 and CAC0110 Sulfate adenylyltransferase (SAT)
High CAC0973 Argininosuccinate synthase
Secondary (K=2) Low   No core set
Med   No core set
High CAC0316 Ornithine carbamoyltransferase
CAC2389 Acetylglutamate kinase
Tertiary (K=3) Low   No core set
Med   No core set
High CAC0974 Argininosuccinate lyase
Quaternary (K=4) Low   No core set
Med   No core set
High [minimal effect on biomass] CAC2391 or CAC3020 4-aminobutyrate aminotransferase acetylornithine/N-succinyldiaminopimelate aminotransferase
  1. If regulatory bounds were imposed exclusively on the reactions in the core set, the flux ranges would match those of the model with all regulatory constraints. Regulation was removed from the primary core sets to obtain secondary cores sets, and so on. Biomass regulation reduced with increasing order of core sets.