Capturing the response of Clostridium acetobutylicumto chemical stressors using a regulated genome-scale metabolic model

Table 6 Genes associated with core set of reactions under butyrate stress conditions

	Stress level	ORF	Gene
Primary (K=1)	Low [minimal effect on biomass]	CAC2113	Bifunctional pyrimidine regulatory protein PyrR/uracil phosphoribosyltransferase (UPRT)
	Med [minimal effect on biomass]	CAC0109 and CAC0110	Sulfate adenylyltransferase (SAT)
	High	CAC0973	Argininosuccinate synthase
Secondary (K=2)	Low		No core set
	Med		No core set
	High	CAC0316	Ornithine carbamoyltransferase
	High	CAC2389	Acetylglutamate kinase
Tertiary (K=3)	Low		No core set
	Med		No core set
	High	CAC0974	Argininosuccinate lyase
Quaternary (K=4)	Low		No core set
	Med		No core set
	High [minimal effect on biomass]	CAC2391 or CAC3020	4-aminobutyrate aminotransferase acetylornithine/N-succinyldiaminopimelate aminotransferase

If regulatory bounds were imposed exclusively on the reactions in the core set, the flux ranges would match those of the model with all regulatory constraints. Regulation was removed from the primary core sets to obtain secondary cores sets, and so on. Biomass regulation reduced with increasing order of core sets.

ISSN: 2731-3654