Skip to main content

Table 2 Calibration results for each organic acid

From: Analytical method for the determination of organic acids in dilute acid pretreated biomass hydrolysate by liquid chromatography-time-of-flight mass spectrometry

Compound

Equation

Regression coefficient

LLQ (μg/mL)

ULQ (μg/mL)

LOD (μg/mL)

Oxalic acid

y =8187.5x - 7954.7

0.9988

2.87

28.71

0.50

cis-Aconitic acid

y =21093x - 5565.6

0.9985

0.31

124.00

0.06

Maleic acid

y =84067x - 16233

0.9992

0.27

81.00

0.05

Glucuronic acid

y =14419x +1094.2

0.9993

0.53

15.60

0.11

Citric acid

y =20732x +6991.4

0.9996

0.34

102.00

0.07

Galacturonic acid

y =15778x +7460.2

0.9986

0.72

36.00

0.11

Gluconic acid

y =12550x +6863.1

0.9990

0.29

58.00

0.10

Pyruvic acid

y =14707x +10512

0.9990

0.96

48.00

0.10

Tricarballylic acid

y =26542x +1315

0.9998

0.28

55.44

0.05

Glyoxylic acid

y =4295.9x +1770.2

0.9940

1.09

19.50

0.15

Malic acid

y =32279x - 2606.8

0.9992

0.35

70.00

0.04

Malonic acid

y =29560x - 17152

0.9951

0.82

54.45

0.18

trans-Aconitic acid

y =12279x +5867.4

0.9998

0.71

47.00

0.07

Methylmalonic acid

y =34174x - 869.82

0.9992

0.25

9.90

0.03

Succinic acid

y =26782x +4284.6

0.9987

0.25

24.50

0.03

Glycolic acid

y =18633x +16302

0.9970

0.78

39.11

0.04

Lactic acid

y =23633x +25347

0.9980

0.34

34.17

<0.01

Itaconic acid

y =33879x - 2761.6

0.9993

0.23

22.50

0.10

Glutaric acid

y =34426x - 6184.7

0.9988

0.23

23.27

0.10

Fumaric acid

y =31579x +19428

0.9991

0.49

98.00

0.05

2-Hydroxy-2-methylbutyric acid

y =79616x +936.42

0.9998

0.26

50.96

0.03

Adipic acid

y =29753x - 3138.7

0.9996

0.23

46.00

0.05

Levulinic acid

y =4245.6x +11213

0.9976

1.52

152.00

0.30

2-Furoic acid

y =4437.7x +2071.3

0.9990

2.25

45.00

0.70

  1. Lower limit of quantification (LLQ) was determined as the concentration level with a relative standard deviation (RSD) <10% (n =3). Upper limit of quantification (ULQ) was determined as the upper concentration level at which the calibration started to deviate from a linear response. Limit of detection (LOD) was determined as the resulting concentration for a signal-to-noise (S/N) =3 criterion.