Figure 3

Glycome profiling of Arabidopsis biomass samples before and after incubation with wild-type C.bescii , JWCB005 ( ?pyrFA ), and JWCB010 ( ?pyrFA ?pecABCR ). Sequential cell wall extracts were prepared from biomass recovered after 24 hr of growth at 75°C of the designated C. bescii strains. The cell walls were isolated as alcohol insoluble residues (AIRs) from the designated biomass, and the AIR was extracted sequentially using ammonium oxalate (oxalate), sodium carbonate and potassium hydroxide (1 M KOH and 4 M KOH) as described in the Methods section. The extracts were screened by ELISA using 155 mAbs directed against diverse epitopes present on most plant cell wall glycans (Additional file 1: Table S1). The resulting binding response data are represented as heatmaps with white-red-dark blue scale indicating the strength of the ELISA signal (white, red, and dark-blue colors depict strong, medium, and no binding, respectively). The mAbs are grouped based on the cell wall glycans they recognize as depicted in the panel at the right-hand side of the figure. The actual amounts of materials extracted in each extraction step are depicted as bar graphs at the top of the heatmaps. The data represent the average of two biological replicates. The soluble material in the original growth media was also analyzed (Additional file 1: Figure S3).