Deletion of a gene cluster encoding pectin degrading enzymes in Caldicellulosiruptor bescii reveals an important role for pectin in plant biomass recalcitrance

Table 1 Genes in the predicted pectinase gene cluster in Caldicellulosiruptor bescii

Gene	Predicted gene product	CAZy module architecture	Gene location/size
pecA (Cbes1855)	Pectate disaccharide lyase (EC:4.2.2.9)	SP-CBM_4_9-PL9	1933527-1935488/1.962 kb
pecB (Cbes1854)	Pectate lyase (EC:4.2.2.2)	SP-CBM_4_9-PL3	1932088-1933470/1.383 kb
pecC (Cbes1853)	Rhamnogalacturonan lyase	SP-PL11-CBM3	1929397-1931898/2.502 kb
pecR (Cbes1856)	AraC family transcriptional regulator		1935834-1938215/2.382 kb

Cbes1853, 1854, and 1855 are predicted to encode extracellular CAZy proteins [19] which contain signal peptides at the 5'- end and a CBM. The abbreviations are: CAZy, carbohydrate active enzyme; EC, enzyme commission number; SP, signal peptide; PL3, PL9, and PL11, polysaccharide lyase family of 3, 9, and 11; CBM, carbohydrate-binding module; CBM3, carbohydrate-binding module of family 3; CBM_4_9, putative pectin-binding module [12],[13].

ISSN: 2731-3654