Figure 5

Specific Avicelase activity of purified Tr-Te chimeric cellobiohydrolase I (CBHI). Both chimeric and native CBHI were loaded at 46.7 mg/g cellulose, whereas the “helper enzyme” endoglucanase (EG) from Acidothermus cellulolyticus (that is, E1-CAT) was loaded at 1.9 mg/g cellulose, and β-D-glucosidase (BGL) at 2 mg/g cellulose. Substrate is Avicel PH-101 at 5.0 mg/mL, digestion at 40°C and pH 5.0 in 20 mM acetate. Values are presented as the mean (±SE) of three replicates.