Figure 1From: Engineering geminivirus resistance in Jatropha curcusSchematic diagrams of the transformation vector and experimental procedure. (A) Selection of three DNA fragments targeting different viral genomic regions. (B) The three fragments were ligated and assembled into a sequence which, when transcribed, would produce a hairpin RNAi structure. Arrows indicate the orientation of each fragment in the hairpin structure. (C) Schematic diagram of the structural features of the inducible pX9- hpICMV RNAi construct and Cre/loxP-mediated DNA recombination (Zuo et al. Guo et al. [19],[20]). Cre, the bacteriophage P1 Cre recombinase with an intron; Frag, fragment; Hpt, a hygromycin-resistance marker gene driven by nopaline synthase (nos) promoter (Pnos); ICMV, Indian cassava mosaic virus; loxP, specific recognition sites of Cre; OlexA-46, eight copies of the LexA DNA-binding site fused to the −46 CaMV 35S promoter; XVE, a chimeric transactivator containing the regulator domain of an estrogen receptor. Arrows inside transcription units indicate the direction of transcription. P1 to P4 denote primers used for PCR analysis to a detect recombination event for Hpt selection marker excision shown in Figure 2. (D) A flowchart of experimental procedure for screening virus resistance transgenic events by Agrobacterium-mediated infection.Back to article page