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Table 2 Characteristics of purified enzymes

From: Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass

Enzyme Molecular weight Substrate specificity (U/mg) Thermostability (Tm, °C)
(apparent, k)
SDS-PAGE Gel filtration Avicel CMC Birch xylan PNP-lactose PNP-glucose PNP-xylose
Bgl3A 82.1 92.8 ND ND 0.44 ND 10.70 0.10 66.0
Cel5A 50.4 61.6 0.26 17.70 ND ND 0.05 ND 77.0
Cel6A 56.3 82.2 0.29 ND 0.87 ND ND ND 63.0
Cel7A 59.5 60.3 0.25 ND ND 0.14 ND ND 67.5
Cel7B 60.9 80.1 0.23 16.15 1.88 1.19 ND ND 64.0
Xyl10A 49.8 44.1 ND 0.91 95.08 0.96 ND 0.31 82.0
  1. Tm values were determined by fluorescence-based thermal shift assay as described in the Methods. CMC, carboxymethyl cellulose; ND, not detectable; PNP-glucose, p-nitrophenyl-β-d-glucoside; PNP-lactose, p-nitrophenyl-β-d-lactoside; PNP-xylose, p-nitrophenyl-β-d-xyloside.