Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass

Table 2 Characteristics of purified enzymes

Enzyme	Molecular weight		Substrate specificity (U/mg)						Thermostability (T_m, °C)
	(apparent, k)		Substrate specificity (U/mg)
	SDS-PAGE	Gel filtration	Avicel	CMC	Birch xylan	PNP-lactose	PNP-glucose	PNP-xylose
Bgl3A	82.1	92.8	ND	ND	0.44	ND	10.70	0.10	66.0
Cel5A	50.4	61.6	0.26	17.70	ND	ND	0.05	ND	77.0
Cel6A	56.3	82.2	0.29	ND	0.87	ND	ND	ND	63.0
Cel7A	59.5	60.3	0.25	ND	ND	0.14	ND	ND	67.5
Cel7B	60.9	80.1	0.23	16.15	1.88	1.19	ND	ND	64.0
Xyl10A	49.8	44.1	ND	0.91	95.08	0.96	ND	0.31	82.0

T_m values were determined by fluorescence-based thermal shift assay as described in the Methods. CMC, carboxymethyl cellulose; ND, not detectable; PNP-glucose, p-nitrophenyl-β-d-glucoside; PNP-lactose, p-nitrophenyl-β-d-lactoside; PNP-xylose, p-nitrophenyl-β-d-xyloside.

ISSN: 2731-3654