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Table 4 Enzymatic hydrolysis of pretreated corn stover (PCS) by various mixtures of core cellulolytic enzymes

From: Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass

Combination number Proportion of enzyme mixture PCS glucan hydrolysis (%)
Cel7A Cel6A Cel5A Xyl10A Average SD
1 0.325 0.325 0.175 0.175 38.6 0.4
2 0.700 0.200 0.050 0.050 39.5 0.0
3 0.200 0.700 0.050 0.050 34.9 0.2
4 0.200 0.200 0.550 0.050 31.8 0.0
5 0.200 0.200 0.050 0.550 29.3 0.2
6 0.450 0.450 0.050 0.050 39.3 0.3
7 0.450 0.200 0.300 0.050 39.6 0.3
8 0.450 0.200 0.050 0.300 36.5 0.2
9 0.200 0.450 0.300 0.050 35.4 0.2
10 0.200 0.450 0.050 0.300 33.5 0.4
11 0.200 0.200 0.300 0.300 32.3 0.6
12 0.512 0.263 0.113 0.113 41.6 0.2
13 0.263 0.512 0.113 0.113 38.0 0.4
14 0.263 0.263 0.362 0.113 36.6 0.1
15 0.263 0.263 0.113 0.362 34.3 0.5
  1. Hydrolysis was carried out at pH 5, 45°C, with 3% (w/v) PCS and a protein loading of 2.55 Mg/g glucan, consisting of 40 μg of core enzyme mixture (Cel7A, Cel6A, Cel5A, and Xyl10A) and 5 μg of Bgl3A in a final volume of 1 ML. Percent hydrolysis of PCS glucan was calculated from the amount of glucose in the hydrolysate after 48 hours. SD, standard deviation.