Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass

Table 4 Enzymatic hydrolysis of pretreated corn stover (PCS) by various mixtures of core cellulolytic enzymes

Combination number	Proportion of enzyme mixture				PCS glucan hydrolysis (%)
Combination number	Cel7A	Cel6A	Cel5A	Xyl10A	Average	SD
1	0.325	0.325	0.175	0.175	38.6	0.4
2	0.700	0.200	0.050	0.050	39.5	0.0
3	0.200	0.700	0.050	0.050	34.9	0.2
4	0.200	0.200	0.550	0.050	31.8	0.0
5	0.200	0.200	0.050	0.550	29.3	0.2
6	0.450	0.450	0.050	0.050	39.3	0.3
7	0.450	0.200	0.300	0.050	39.6	0.3
8	0.450	0.200	0.050	0.300	36.5	0.2
9	0.200	0.450	0.300	0.050	35.4	0.2
10	0.200	0.450	0.050	0.300	33.5	0.4
11	0.200	0.200	0.300	0.300	32.3	0.6
12	0.512	0.263	0.113	0.113	41.6	0.2
13	0.263	0.512	0.113	0.113	38.0	0.4
14	0.263	0.263	0.362	0.113	36.6	0.1
15	0.263	0.263	0.113	0.362	34.3	0.5

Hydrolysis was carried out at pH 5, 45°C, with 3% (w/v) PCS and a protein loading of 2.55 Mg/g glucan, consisting of 40 μg of core enzyme mixture (Cel7A, Cel6A, Cel5A, and Xyl10A) and 5 μg of Bgl3A in a final volume of 1 ML. Percent hydrolysis of PCS glucan was calculated from the amount of glucose in the hydrolysate after 48 hours. SD, standard deviation.

ISSN: 2731-3654