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Table 1 M. extorquens strains and plasmids used in this study

From: Metabolic engineering of Methylobacterium extorquens AM1 for 1-butanol production

Strain Plasmid/Genotype* Reference
Wild type Rif derivative 27
BHB1 pCM66 (Plac:: bcd&etf-adhE2) This study
BHB2 pCM80 (PmxaF:: bcd&etf-adhE2) This study
BHB3 pAP775 (Pmeta1_3616 :: bcd&etf-adhE2) This study
BHB4 pCM80 (PmaxF:: adhE2-ter) This study
BHB5 pCM66 (Plac:: adhE2-ter) This study
BHB6 pAP776 (Pmeta1_002:: adhE2-ter) This study
BHB7 pAP775 (Pmeta1_3616 :: adhE2-ter) This study
BHB8 pAP775 (Pmeta1_3616 :: ter-adhE2) This study
BHB9 pHC61 (Pmtac:: croR:: Pmeta1_3616 :: adhE2-ter) This study
Plasmids Description Reference
pCM66 M. extorquens expression vector (Plac, KmR) 25
pCM80 M. extorquens expression vector (PmxaF, TcR) 25
pAP775 M. extorquens expression vector (Pmeta1_3616, TcR) E. Skovran
pAP776 M. extorquens expression vector (Pmeta1_002, TcR) E. Skovran
pHC61 M. extorquens expression vector (Pmtac, KmR) 47
  1. *Promoter strength: PmxaF > Pmeta1_3616 > Pmeta1_002 > Plac.