Degradation of wheat straw by P. chrysosporium with different incubation periods (A), chemical compositional structural analysis (B), and enzymatic hydrolysis (C). Sterilized wheat straw samples (100 g) were treated in plastic containers inside a low temperature 815 incubator (Precision Scientific) (A). The containers were covered with eight-layer gauze pads in order to allow air exchange. Every 48 to 72 hours, 50 mL of water was evenly sprayed on the surface of the wheat straw while manually rotating the samples. At the end of the incubation periods, each group was collected individually, washed with water, dried, and milled to a fine powder (44 to 74 um).