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Figure 2 | Biotechnology for Biofuels

Figure 2

From: Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme

Figure 2

Electrotransformation data demonstrating that Clostridium pasteurianum restricts a 334 bp region within the ltrA gene sequence of pSY6catP. The 932 bp SacII-BstAPI region of pSY6catP (see Figure 1) is enlarged to better show relevant vector components. Point mutations within the ltrA coding sequence are depicted as vertical bands. Enlarged vector components are depicted to scale. Shaded box: Ll.ltrB intron; shaded arrow: ltrA; dashed line: Dcm recognition site; Pptb: ptb promoter (C. acetobutylicum); ND: not detected. Relevant restriction endonuclease recognition sites corresponding to AatII (A), BglII (B), EcoO109I (E), and SacII (S) are abbreviated using a single letter.

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