Electrotransformation results demonstrating successful electrotransformation of Δ cpaAIR gene disruption cells with M.FnuDII-unmethylated plasmid pMTL85141ermB. Wild-type cells (top row) and ΔcpaAIR gene disruption cells (bottom row) of Clostridium pasteurianum were electroporated separately with M.FnuDII-unmethylated (left column) and M.FnuDII-methylated (right column) plasmid pMTL85141ermB. M.FnuDII methylation was achieved in vivo using an Escherichia coli strain harboring pMTL85141ermB and pFnuDIIMKn. Varying volumes of electrotransformation outgrowth cell suspensions were plated to give approximately equal numbers of transformants between electrotransformations. Hence, the number of transformant colonies shown does not allow for a direct comparison of electrotransformation efficiency.