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Table 4 Specific activity (μmol/min/mg protein) of putative T. pseudethanolicus 39E aldehyde reductases

From: A comparative multidimensional LC-MS proteomic analysis reveals mechanisms for furan aldehyde detoxification in Thermoanaerobacter pseudethanolicus 39E

 

Vector control

Teth39 BdhA

Teth39 AKR

Furfural

0.33 ± 0.13

4.97 ± 0.17

0.27 ± 0.04

5-Hydroxymethylfurfural

0.44 ± 0.16

10.06 ± 0.80

0.39 ± 0.07

Acetaldehyde

0.02 ± 0.15

4.54 ± 0.76

0.02 ± 0.01

Butyraldehyde

0.09 ± 0.05

14.58 ± 3.57

0.33 ± 0.04

Isobutyraldehyde

−0.10 ± 0.21

0.63 ± 0.29

0.23 ± 0.05

  1. Whole cell lysate with pET-30a only (vector control) or expressing Teth39 BdhA (Teth39_1597) or Teth39 AKR (Teth39_1598) was assayed aerobically at 60°C using 0.2 mM NAD(P)H as the electron donor and 20 mM substrate. NAD(P)H oxidation activity was measured via loss of absorbance at 340 nm. Values in boldface are statistically significant. No activity was detected with either Teth39 BdhA or Teth39 AKR above vector control using NADH.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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